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11 March 2020 Non-invasive intracellular dynamics study using quadriwave lateral shearing interferometry (Conference Presentation)
Roman Zinchuk, Julien Savatier, Anaïs Saintoyant, Sherazade Aknoun, Antoine Federici, Serge Monneret, Benoit Wattellier
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Proceedings Volume 11249, Quantitative Phase Imaging VI; 112490W (2020) https://doi.org/10.1117/12.2544711
Event: SPIE BiOS, 2020, San Francisco, California, United States
Abstract
We propose to study the behavior of vesicles at the intracellular scale with a non-invasive quantitative phase imaging technology which enables to follow vesicles during a large amount of time without modifying their behavior. We developed specific tools to localize and track cells that we compare to fluorescence-based tracking method. We studied their interaction with the cytoskeleton by inhibiting it using drugs and we implemented statistical tools to quantify and understand intracellular processes at the vesicular level. The resolution and the high sensitivity brought by the High Definition wave front sensor we developed enables to follow all individual vesicles through time follow up, while imaging the whole cell.
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Roman Zinchuk, Julien Savatier, Anaïs Saintoyant, Sherazade Aknoun, Antoine Federici, Serge Monneret, and Benoit Wattellier "Non-invasive intracellular dynamics study using quadriwave lateral shearing interferometry (Conference Presentation)", Proc. SPIE 11249, Quantitative Phase Imaging VI, 112490W (11 March 2020); https://doi.org/10.1117/12.2544711
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KEYWORDS
Interferometry
Algorithm development
Cytoskeletons
Detection and tracking algorithms
Phase imaging
Detector development
Image resolution
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