Illumination And Detection Strategies For Confocal Microscopy

G. J. Brakenhoff; H T. M. van der Voort

doi:10.1117/12.961783

28 September 1989 Illumination And Detection Strategies For Confocal Microscopy

G. J. Brakenhoff, H T. M. van der Voort

Proceedings Volume 1139, Optical Storage and Scanning Technology; (1989) https://doi.org/10.1117/12.961783
Event: 1989 International Congress on Optical Science and Engineering, 1989, Paris, France

Abstract

A confocal microscope can be considered as a 3-D sampling instrument to collect data from spatial structures, especially biological ones. Optimal performance requires the adaptation of the dimensions of the sampling volume to the lateral and axial raster parameters employed during data collection. It will be shown that the shape of the sampling volume can be controlled through optical means by a combination of specific illumination and detection parameters. The use of these techniques in computer controlled instruments is discussed, especially in relation to operation in fluorescence.

Citation Download Citation

G. J. Brakenhoff and H T. M. van der Voort "Illumination And Detection Strategies For Confocal Microscopy", Proc. SPIE 1139, Optical Storage and Scanning Technology, (28 September 1989); https://doi.org/10.1117/12.961783

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