Untethering single molecule spectroscopy with 3D-SMART

Shangguo Hou; Jack Exell; Kevin Welsher

doi:10.1117/12.2578699

5 March 2021 Untethering single molecule spectroscopy with 3D-SMART

Shangguo Hou, Jack Exell, Kevin Welsher

Proceedings Volume 11650, Single Molecule Spectroscopy and Superresolution Imaging XIV; 116500L (2021) https://doi.org/10.1117/12.2578699
Event: SPIE BiOS, 2021, Online Only

Abstract

Measuring the behavior of single molecules enables the discovery of states and dynamics obscured by bulk measurements. However, molecules in solution rapidly diffuse in three dimensions, precluding long-duration and high-temporal resolution measurement. To overcome this hurdle, we have developed 3D single-molecule active real-time tracking (3D-SMART) which enables active feedback tracking of rapidly diffusing (exceeding 10 μm2/sec) and lowly emitting fluorescent (rates of 10 kHz or less) particles. Here we demonstrate the application of 3D-SMART to a range targets, from single virus-like particles and quantum dots in water, all the way down to single fluorophores in viscous solution. This new single molecule tracking can be applied to continuously monitor single proteins and nucleic acids, including real-time measurement of transcription on a freely diffusing, single-dye labelled DNA strand. 3D-SMART represents a critical step towards the untethering of single molecule spectroscopy.

Conference Presentation

Citation Download Citation

Shangguo Hou, Jack Exell, and Kevin Welsher "Untethering single molecule spectroscopy with 3D-SMART", Proc. SPIE 11650, Single Molecule Spectroscopy and Superresolution Imaging XIV, 116500L (5 March 2021); https://doi.org/10.1117/12.2578699

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KEYWORDS

Single molecule spectroscopy

3D metrology

Molecules

Atmospheric particles

3D acquisition

Laser development

Molecular spectroscopy

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