Super-resolution imaging of amyloid-β plaques and autofluorescence suppression in mouse brains

Daiki Hara; Yuki Shimaoka; Shin-nosuke Uno; Masaya Okada; Shigeki Iwanaga

doi:10.1117/12.2578140

5 March 2021 Super-resolution imaging of amyloid-β plaques and autofluorescence suppression in mouse brains

Daiki Hara, Yuki Shimaoka, Shin-nosuke Uno, Masaya Okada, Shigeki Iwanaga

Proceedings Volume 11650, Single Molecule Spectroscopy and Superresolution Imaging XIV; 116500R (2021) https://doi.org/10.1117/12.2578140
Event: SPIE BiOS, 2021, Online Only

Conference Poster

Abstract

Abnormal deposition of amyloid-β (Aβ) causes the formation of senile plaques, which is one of the main pathological features of Alzheimer disease. To visualize the plaques at nanometer resolution, single-molecule localization microscopy (SMLM) is promising. It is also essential to develop a method for suppressing autofluorescence especially under high-magnifications used for detecting single molecules. Here, we report a novel method to reduce autofluorescence in mouse brains which is applicable to visualize the structure of Aβ plaques by SMLM. The super-resolution images of Aβ plaques showed fibrous structures that were not able to be discerned by conventional fluorescence imaging.

Conference Presentation

Citation Download Citation

Daiki Hara, Yuki Shimaoka, Shin-nosuke Uno, Masaya Okada, and Shigeki Iwanaga "Super-resolution imaging of amyloid-β plaques and autofluorescence suppression in mouse brains", Proc. SPIE 11650, Single Molecule Spectroscopy and Superresolution Imaging XIV, 116500R (5 March 2021); https://doi.org/10.1117/12.2578140

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