Hybridization of quantitative phase microscopy and fluorescent microscopy in measurements of cells under osmotic stress

Almog Taieb; Itay Barnea; Natan Tzvi Shaked

doi:10.1117/12.2577827

5 March 2021 Hybridization of quantitative phase microscopy and fluorescent microscopy in measurements of cells under osmotic stress

Almog Taieb, Itay Barnea, Natan Tzvi Shaked

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Proceedings Volume 11655, Label-free Biomedical Imaging and Sensing (LBIS) 2021; 116550U (2021) https://doi.org/10.1117/12.2577827
Event: SPIE BiOS, 2021, Online Only

Abstract

We propose a multimodal quantitative imaging technique by integrating off-axis interferometric phase microscopy (IPM) and fluorescence microscopy, for measuring morphological changes of cells induced by hyper-osmotic pressure. Based on the collected data, we calculated the temporal dependence of various physiological parameters during osmotic pressure induced by the cell surrounding-medium change. This technique is expected to be a useful for observing the dynamics of live cells, while obtaining both quantitative imaging capabilities and molecular specificity of specific cellular organelles.

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Almog Taieb, Itay Barnea, and Natan Tzvi Shaked "Hybridization of quantitative phase microscopy and fluorescent microscopy in measurements of cells under osmotic stress", Proc. SPIE 11655, Label-free Biomedical Imaging and Sensing (LBIS) 2021, 116550U (5 March 2021); https://doi.org/10.1117/12.2577827

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KEYWORDS

Microscopy

Interferometry

Multimodal imaging

Green fluorescent protein

Holograms

Live cell imaging

Luminescence

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