Optical and molecular longitudinal tracking of primary colorectal murine tumors shows differences in the angiogenic response to maximum-tolerated and metronomic approaches

Ariel I. Mundo; Abdussaboor Muhammad; Timothy J. Muldoon

doi:10.1117/12.2576906

5 March 2021 Optical and molecular longitudinal tracking of primary colorectal murine tumors shows differences in the angiogenic response to maximum-tolerated and metronomic approaches

Ariel I. Mundo, Abdussaboor Muhammad, Timothy J. Muldoon

Author Affiliations +

Proceedings Volume 11655, Label-free Biomedical Imaging and Sensing (LBIS) 2021; 116551C (2021) https://doi.org/10.1117/12.2576906
Event: SPIE BiOS, 2021, Online Only

Abstract

The current paradigm for chemotherapy in the treatment of colorectal cancer (CRC) is based on the maximum-tolerated dose approach (MTD), which uses high doses of cytotoxic agents with the aim of killing tumor cells. However, the toxicity of MTD chemotherapy requires drug delivery in a cyclic manner with rest periods that allow the tumor to recover. In contrast, metronomic chemotherapy (MET) uses low but continuous doses that target the endothelial cells of tumors, thereby directly affecting the development of new blood vessels (angiogenesis). Because the potential impact of MET when used as neoadjuvant chemotherapy (NAC) in CRC has not been explored and contrasted with the effects in perfusion of the standard MTD regimen, this study uses an azoxymethane-induced primary model of CRC to assess changes in perfusion and angiogenic markers. Diffuse reflectance spectroscopy (DRS) was used to longitudinally quantify oxygen saturation (StO2) and compare these changes with the change in expression of VEGF-A between MET and MTD treatments. In the MTD group, an increase in StO2 from 65 to 81% was observed by week 4 of treatment and a reduction of VEGF-A expression from 81% to 16% was observed between weeks 4 and 6.

Conference Presentation

Citation Download Citation

Ariel I. Mundo, Abdussaboor Muhammad, and Timothy J. Muldoon "Optical and molecular longitudinal tracking of primary colorectal murine tumors shows differences in the angiogenic response to maximum-tolerated and metronomic approaches", Proc. SPIE 11655, Label-free Biomedical Imaging and Sensing (LBIS) 2021, 116551C (5 March 2021); https://doi.org/10.1117/12.2576906

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