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5 March 2021 Interferometric detection of scattering (iSCAT): from sensing to microscopy
Vahid Sandoghdar
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Proceedings Volume 11657, Biomedical Applications of Light Scattering XI; 1165705 (2021) https://doi.org/10.1117/12.2583829
Event: SPIE BiOS, 2021, Online Only
Abstract
While fluorescence labeling remains a powerful tool for many studies, it also poses fundamental limitations, which have motivated many groups to develop fluorescence-free measurement methods. Scattering offers many interesting opportunities for these efforts, but lack of spectral specificity makes the detection of small objects via scattering challenging. Nearly two decades ago, we showed that, nevertheless, single gold nanoparticles as small as 5 nm could be detected via interferometric detection, giving birth to a new detection scheme that was later coined iSCAT. Since then, it has been shown that small unlabeled proteins could be detected and counted, and transmembrane proteins tracked on living cells using gold nanoparticles as scattering label. In this presentation, I discuss the technical subtleties of iSCAT, its principal advantages and several new studies that have become possible by it.
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Vahid Sandoghdar "Interferometric detection of scattering (iSCAT): from sensing to microscopy", Proc. SPIE 11657, Biomedical Applications of Light Scattering XI, 1165705 (5 March 2021); https://doi.org/10.1117/12.2583829
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KEYWORDS
Scattering
Microscopy
Interferometry
Nanoparticles
Proteins
Gold
Rayleigh scattering
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