Using light to establish and measure stiffness gradients in three-dimensional engineered tissues

Alicja Jagiello; Qingda Hu; Ulysses Castillo; Elliot Botvinick

doi:10.1117/12.2595590

3 August 2021 Using light to establish and measure stiffness gradients in three-dimensional engineered tissues

Alicja Jagiello, Qingda Hu, Ulysses Castillo, Elliot Botvinick

Proceedings Volume 11798, Optical Trapping and Optical Micromanipulation XVIII; 117980A (2021) https://doi.org/10.1117/12.2595590
Event: SPIE Nanoscience + Engineering, 2021, San Diego, California, United States

Abstract

Studies of cell-extracellular matrix (ECM) interactions within fibrous systems such as collagen or fibrin are challenging, particularly if peri-cellular stiffness cannot be monitored. Here we present our light-based method for non-invasive patterning of molecular crosslinking combined with multi-axes optical tweezers active microrheology to map ECM stiffness landscapes. This method allows us to generate prescribed stiffness gradients and associated anisotropies, which model stiffness of the natural peri-cellular ECM. Patterned crosslinking induces strain hardening and measured stiffness gradients are in agreement with predicted strain fields. Migratory cells respond to these gradients as assessed by change in F-actin distribution and morphological properties.

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Citation Download Citation

Alicja Jagiello, Qingda Hu, Ulysses Castillo, and Elliot Botvinick "Using light to establish and measure stiffness gradients in three-dimensional engineered tissues", Proc. SPIE 11798, Optical Trapping and Optical Micromanipulation XVIII, 117980A (3 August 2021); https://doi.org/10.1117/12.2595590

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KEYWORDS

Tissues

Anisotropy

3D modeling

Collagen

Optical fibers

Optical lithography

Optical tweezers

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