FDISCO+: a clearing method for robust fluorescence preservation of cleared samples

Peng Wan; Yusha Li; Jingtan Zhu; Jianyi Xu; Xiaomei Liu; Tingting Yu; Dan Zhu

doi:10.1117/12.2601107

9 October 2021 FDISCO+: a clearing method for robust fluorescence preservation of cleared samples

Peng Wan, Yusha Li, Jingtan Zhu, Jianyi Xu, Xiaomei Liu, Tingting Yu, Dan Zhu

Proceedings Volume 11900, Optics in Health Care and Biomedical Optics XI; 119001Y (2021) https://doi.org/10.1117/12.2601107
Event: SPIE/COS Photonics Asia, 2021, Nantong, JS, China

Conference Poster

Abstract

The recently reported solvent-based optical clearing method FDISCO can preserve various fluorescent signals very well. However, the strict low-temperature storage condition of FDISCO is not conducive to long-time or repetitive imaging usually conducted at room temperature (RT). Therefore, it is important to solve the contradiction between fluorescence preservation and imaging condition. We develop a modified FDISCO clearing method, termed FDISCO+, to change the preservation condition from low temperature (LT) to RT. Two alternative antioxidants were screened out to effectively inhibit the peroxide generation in the clearing agent at RT, enabling robust fluorescence preservation of cleared samples. FDISCO+ achieves comparable fluorescence preservation with the original FDISCO protocol and allows long-time storage at RT, making it easier for researchers to image and preserve the samples. FDISCO+ is expected to be widely used due to its loose operation requirements.

Citation Download Citation

Peng Wan, Yusha Li, Jingtan Zhu, Jianyi Xu, Xiaomei Liu, Tingting Yu, and Dan Zhu "FDISCO+: a clearing method for robust fluorescence preservation of cleared samples", Proc. SPIE 11900, Optics in Health Care and Biomedical Optics XI, 119001Y (9 October 2021); https://doi.org/10.1117/12.2601107

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