Simultaneous light-field fluorescence and TIE-based phase imaging

Sudheesh K. Rajput; Ryo Shinke; Xiangyu Quan; Yasuhiro Awatsuji; Osamu Matoba

doi:10.1117/12.2615674

27 October 2021 Simultaneous light-field fluorescence and TIE-based phase imaging

Sudheesh K. Rajput, Ryo Shinke, Xiangyu Quan, Yasuhiro Awatsuji, Osamu Matoba

Proceedings Volume 11925, Biomedical Imaging and Sensing Conference 2021; 119250S (2021) https://doi.org/10.1117/12.2615674
Event: SPIE Technologies and Applications of Structured Light, 2021, Online, Japan

Abstract

In this paper, we integrate the fluorescence and phase imaging simultaneously that uses the light field and the transport of intensity equation (TIE). Here, the fluorescence information is obtained by the light field imaging with an electrically tunable lens while phase imaging is done by TIE. For fluorescence, different intensity stacks are captured with image sensor to be used for reconstructing the fluorescence images using the concept of light field. To get the phase information of the same sample along with fluorescence, the three intensity images are captured by another image sensor for solving TIE for phase data. Experimental results of micro beads are presented in support of simultaneous imaging technique.

Citation Download Citation

Sudheesh K. Rajput, Ryo Shinke, Xiangyu Quan, Yasuhiro Awatsuji, and Osamu Matoba "Simultaneous light-field fluorescence and TIE-based phase imaging", Proc. SPIE 11925, Biomedical Imaging and Sensing Conference 2021, 119250S (27 October 2021); https://doi.org/10.1117/12.2615674

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