IR microfluidics for in situ sensing of molecular interfaces

Karsten Hinrichs; Jörg Rappich; Andreas Furchner; Christoph Kratz

doi:10.1117/12.2608582

2 March 2022 IR microfluidics for in situ sensing of molecular interfaces

Karsten Hinrichs, Jörg Rappich, Andreas Furchner, Christoph Kratz

Proceedings Volume 11953, Optical Fibers and Sensors for Medical Diagnostics, Treatment and Environmental Applications XXII; 119530B (2022) https://doi.org/10.1117/12.2608582
Event: SPIE BiOS, 2022, San Francisco, California, United States

Abstract

Recent developments in infrared (IR) microfluidics for sensitive monitoring of molecular adsorption at solid–liquid interfaces are briefly reviewed. A microfluidic platform is presented that uses a metallic island film for surface enhanced IR absorption (SEIRA) coupled to IR spectroscopies for bio-sensing and vibrational investigations of molecular monolayers and their adsorption kinetics. Exemplarily, IR spectral monitoring of the monolayer formation of 4-mercaptobenzonitrile (4-MBN) in liquid environment is discussed as a Langmuir isotherm. Adsorption isotherms of specific molecular vibrations are analyzed from the time-dependent evolution of band amplitudes and peak areas during adsorption. Given the detection limit of 0.03 nmol/cm2 , the isotherms of 4-MBN, gluthathione (GSH) monolayer formation, and the sensing of 4-nitrobenzylmercaptan (4-NBM) by the MP/graphene surface are compared. Potential applications are bio- and bio-medical sensing as well as the study of processes, e. g., enzymatic reactions, chemical or catalytic reactions, receptor–ligand interactions, and structural changes of molecules due to environmental stimuli.

Conference Presentation

Citation Download Citation

Karsten Hinrichs, Jörg Rappich, Andreas Furchner, and Christoph Kratz "IR microfluidics for in situ sensing of molecular interfaces", Proc. SPIE 11953, Optical Fibers and Sensors for Medical Diagnostics, Treatment and Environmental Applications XXII, 119530B (2 March 2022); https://doi.org/10.1117/12.2608582

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