Designing a quantifiable detection method for the optimization of gold nanoparticle based gene therapy

Jacqueline Labovitz; Menachem Motiei; Tamar Sadan; Dror Fixler; Rachela Popovtzer

doi:10.1117/12.2608944

3 March 2022 Designing a quantifiable detection method for the optimization of gold nanoparticle based gene therapy

Jacqueline Labovitz, Menachem Motiei, Tamar Sadan, Dror Fixler, Rachela Popovtzer

Proceedings Volume 11976, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XIX; 119760C (2022) https://doi.org/10.1117/12.2608944
Event: SPIE BiOS, 2022, San Francisco, California, United States

Conference Poster

Abstract

Gold nanoparticles (GNPs) are becoming an increasingly prominent biomedical tool. GNPs are biocompatible and can carry high payloads and a wide array of biological materials, making them an ideal delivery vector for various therapeutics, such as gene therapy. However, one major obstacle to clinical application is endosomal entrapment and subsequent degradation of the nanoparticletherapy complex. Coating GNPs with an endosomal escape agent can serve as an effective approach to overcome this challenge. This study explores the probability of different types of coated GNPs to perform endosomal escape. We used a novel, multi-modal approach applying fluorescent confocal microscopy, as well as sophisticated image analysis, to provide a quantitative and uniform method that can denote endosomal escape efficacy. Our findings can ultimately advance understanding of endosomal escape abilities of various GNP coatings and promote their application for gene therapy.

Citation Download Citation

Jacqueline Labovitz, Menachem Motiei, Tamar Sadan, Dror Fixler, and Rachela Popovtzer "Designing a quantifiable detection method for the optimization of gold nanoparticle based gene therapy", Proc. SPIE 11976, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XIX, 119760C (3 March 2022); https://doi.org/10.1117/12.2608944

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