Fluorescence decay time imaging using an imaging photon detector with a radio frequency photon correlation system

Christopher G. Morgan; A. C. Mitchell; J. G. Murray

doi:10.1117/12.17773

1 May 1990 Fluorescence decay time imaging using an imaging photon detector with a radio frequency photon correlation system

Christopher G. Morgan, A. C. Mitchell, J. G. Murray

Author Affiliations +

Proceedings Volume 1204, Time-Resolved Laser Spectroscopy in Biochemistry II; (1990) https://doi.org/10.1117/12.17773
Event: OE/LASE '90, 1990, Los Angeles, CA, United States

Abstract

An imaging photon detector has been modified to incorporate fast timing electronics coupled to a custom built photon correlator interfaced to a RISC computer. Using excitation with intensity- muodulated light, fluorescence images can be readily obtained where contrast is determined by the decay time of emission, rather than by intensity. This technology is readily extended to multifrequency phase/demodulation fluorescence imaging or to differential polarised phase fluorometry. The potential use of the correlator for confocal imaging with a laser scanner is also briefly discussed.

Citation Download Citation

Christopher G. Morgan, A. C. Mitchell, and J. G. Murray "Fluorescence decay time imaging using an imaging photon detector with a radio frequency photon correlation system", Proc. SPIE 1204, Time-Resolved Laser Spectroscopy in Biochemistry II, (1 May 1990); https://doi.org/10.1117/12.17773

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