Influence of end-to-end diffusion on intramolecular energy transfer as observed by frequency-domain fluorometry

Joseph R. Lakowicz; Wieslaw M. Wiczk; Ignacy Gryczynski; Henryk Szmacinski; Michael L. Johnson

doi:10.1117/12.17748

1 May 1990 Influence of end-to-end diffusion on intramolecular energy transfer as observed by frequency-domain fluorometry

Joseph R. Lakowicz, Wieslaw M. Wiczk, Ignacy Gryczynski, Henryk Szmacinski, Michael L. Johnson

Author Affiliations +

Proceedings Volume 1204, Time-Resolved Laser Spectroscopy in Biochemistry II; (1990) https://doi.org/10.1117/12.17748
Event: OE/LASE '90, 1990, Los Angeles, CA, United States

Abstract

We investigated the influence of end-to-end diffusion on intramolecular energy transfer between a naphthalene donor and dansyl acceptor linked by polymethylene chain. A range of viscosities of 0.6 - 200cP were obtained using propylene glycol at different temperatures (0-80°C) and methanol at 20°C. The intensity decays of naphthalene were measured in frequency-domain. Several theoretical models, including distance distributions were used to fit the data. The results indicate that end-to-end diffusion of flexible donor - acceptor pairs can be readily detected and quantified using frequency-domain fluorometry.

Citation Download Citation

Joseph R. Lakowicz, Wieslaw M. Wiczk, Ignacy Gryczynski, Henryk Szmacinski, and Michael L. Johnson "Influence of end-to-end diffusion on intramolecular energy transfer as observed by frequency-domain fluorometry", Proc. SPIE 1204, Time-Resolved Laser Spectroscopy in Biochemistry II, (1 May 1990); https://doi.org/10.1117/12.17748

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available