Dark-field imaging and Raman spectroscopy study of the interaction process between cells and nanoparticles

Zhenhao Mo; Yanhua Zou; Junming Lin; Rui Hu

doi:10.1117/12.2605786

1 November 2021 Dark-field imaging and Raman spectroscopy study of the interaction process between cells and nanoparticles

Zhenhao Mo, Yanhua Zou, Junming Lin, Rui Hu

Author Affiliations +

Proceedings Volume 12057, Twelfth International Conference on Information Optics and Photonics; 1205728 (2021) https://doi.org/10.1117/12.2605786
Event: Twelfth International Conference on Information Optics and Photonics, 2021, Xi'an, China

Abstract

Anisotropic plasmonic gold nanorods (AuNRs) not only have excellent electrical conductivity and electrocatalytic activity, due to localized surface plasmon resonance, they also have good optical properties, such as tunable UV-vis-NIR absorption and so on. Therefore, AuNRs have very high potential in sensing, catalysis, and theranostics. In this contribution, we synthesized AuNR@Ag core-shell metallic nanostructures, with 1,4-benzenedithiol (1,4-BDT) sandwiched between the Au-Ag gaps. To the outside of the covering silver layer, 4-Mercaptopyridine (4-MPY) is attached to give a second Raman signal and a layer of BSA coating is applied for further stability. The nanocomposite is used for cell imaging, whereas the Raman signal from the 4-MPY is used to monitor the effects of the subcellular organelles on the engulfed nanomaterials. Our results show that as the thickness of the silver shell layer increases, the BDT Raman signal intensity gradually increases. When the silver layer thickness exceeds a certain value, the increase in the silver layer thickness will cause the surface enhanced Raman scattering (SERS) signal intensity to drop. And as 4-MPY is embedded on the outside of the silver layer, the Raman spectrum of the entire material adds three new Raman peaks at 428 cm^-1 , 707 cm^-1 , and 1000 cm^-1 , respectively. The changes in these peaks can be used to probe the interactions between cells and the nanomaterials. In addition, the combination of dark-field imaging and Raman spectroscopy for the analysis of the nanomaterial-cell interaction indicates that the SERS nanoprobe can indeed explore the interactions between the cells and nanomaterials. Therefore, these results indicate that the nanoprobe is a cell trace analysis tool with important application prospects.

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Zhenhao Mo, Yanhua Zou, Junming Lin, and Rui Hu "Dark-field imaging and Raman spectroscopy study of the interaction process between cells and nanoparticles", Proc. SPIE 12057, Twelfth International Conference on Information Optics and Photonics, 1205728 (1 November 2021); https://doi.org/10.1117/12.2605786

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KEYWORDS

Raman spectroscopy

Surface enhanced Raman spectroscopy

Nanoparticles

Silver

Molecules

Imaging spectroscopy

Nanomaterials

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