Common-path grating-based interferometers with altered temporal and spatial coherence of illumination for quantitative phase microscopy and tomography

M. Trusiak; K. Patorski; P. Zdańkowski; J. Winnik; W. Lipke

doi:10.1117/12.2636220

3 October 2022 Common-path grating-based interferometers with altered temporal and spatial coherence of illumination for quantitative phase microscopy and tomography

M. Trusiak, K. Patorski, P. Zdańkowski, J. Winnik, W. Lipke

Author Affiliations +

Proceedings Volume 12223, Interferometry XXI; 122230G (2022) https://doi.org/10.1117/12.2636220
Event: SPIE Optical Engineering + Applications, 2022, San Diego, California, United States

Abstract

We discuss implementations of a straightforward common-path interferometric system within the microscope setup to allow for quantitative phase contrast imaging. Fringe pattern generation is based on the interference between +1 and -1 diffraction grating orders. Limited angle optical diffraction tomography is realized upon illuminating the sample under varying angles and reconstructing a 3D refractive index distribution via filtered back-projection. Due to equalized optical path of both conjugate +1/-1 beams temporal coherence alteration can be easily implemented with benefits of coherent noise minimization (reducing speckles and parasitic interferences). Presented setups employ single microscope objective in an open-top configuration allowing for comfortable sample operation. Proposed 2D/3D grating-based common-path quantitative phase imaging systems are limited to sparse samples, as +1/-1 interference needs to superimpose object replica and object-free area, however.

Conference Presentation

Citation Download Citation

M. Trusiak, K. Patorski, P. Zdańkowski, J. Winnik, and W. Lipke "Common-path grating-based interferometers with altered temporal and spatial coherence of illumination for quantitative phase microscopy and tomography", Proc. SPIE 12223, Interferometry XXI, 122230G (3 October 2022); https://doi.org/10.1117/12.2636220

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