Research advances of in-situ soil zymography

Zehui Liu; Mengxuan Han; Guo Yu

doi:10.1117/12.2636836

29 April 2022 Research advances of in-situ soil zymography

Zehui Liu, Mengxuan Han, Guo Yu

Proceedings Volume 12247, International Conference on Image, Signal Processing, and Pattern Recognition (ISPP 2022); 1224723 (2022) https://doi.org/10.1117/12.2636836
Event: 2022 International Conference on Image, Signal Processing, and Pattern Recognition, 2022, Guilin, China

Abstract

Soil enzymes play an important role in the material circulation and energy flow of soil ecosystem, and the detection of their activity is the basis for the development of soil enzymes. Traditional soil enzyme activity detection methods can reflect soil enzyme activity, but cannot reflect the real situation of soil enzyme in situ, and distinguish the continuous changes of enzyme activity in time and space. Based on fluorescent substrate, in-situ zymography technology can obtain two-dimensional images of soil enzyme activity distribution in situ, reflect its continuous changes in space from microscale, and distinguish between hot and non-hot areas of soil enzyme activity. It has the advantages of high accuracy, high spatial resolution and high time resolution. In this paper, the working principle and technical advantages of in-situ zymography were summarized, and the development direction of the combination of in-situ zymography and other technologies was prospected, in order to promote the development and application of this technology and provide research direction for the further study of soil enzymology.

Citation Download Citation

Zehui Liu, Mengxuan Han, and Guo Yu "Research advances of in-situ soil zymography", Proc. SPIE 12247, International Conference on Image, Signal Processing, and Pattern Recognition (ISPP 2022), 1224723 (29 April 2022); https://doi.org/10.1117/12.2636836

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KEYWORDS

Soil science

Spatial resolution

Luminescence

Soil contamination

Ecosystems

Environmental sensing

Microorganisms

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