High-frequency photoacoustic microscopy of hematoxylin and eosin stained slides for veterinary oncology applications

Mindaugas Tamošiūnas; Blaž Cugmas D.V.M.; Renaldas Raišutis

doi:10.1117/12.2650516

19 December 2022 High-frequency photoacoustic microscopy of hematoxylin and eosin stained slides for veterinary oncology applications

Mindaugas Tamošiūnas, Blaž Cugmas D.V.M., Renaldas Raišutis

Author Affiliations +

Proceedings Volume 12320, Optics in Health Care and Biomedical Optics XII; 123202C (2022) https://doi.org/10.1117/12.2650516
Event: SPIE/COS Photonics Asia, 2022, Online Only

Conference Poster

Abstract

We tested high-frequency photoacoustic imaging for efficacy in recovering the histopathological structures from hematoxylin and eosin (H&E) stained tissue sections. The results showed that exogenous staining improved the absorption contrast of the histology microscope slides, allowing the detection of adipose tissue structure with a micrometer resolution throughout the tens of micrometer-square area range. The comparative spectroscopic study proved that photoacoustic imaging caused two simultaneously occurring processes: photodegradation of H&E (and formation of stable photoproducts absorbing in the blue spectral region), and ultrasound assisted organic stain removal from the microscope slide sample, reducing the exogenous contrast-distinguishable photoacoustic detection area.

Conference Presentation

Citation Download Citation

Mindaugas Tamošiūnas, Blaž Cugmas D.V.M., and Renaldas Raišutis "High-frequency photoacoustic microscopy of hematoxylin and eosin stained slides for veterinary oncology applications", Proc. SPIE 12320, Optics in Health Care and Biomedical Optics XII, 123202C (19 December 2022); https://doi.org/10.1117/12.2650516

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