Video-rate wide-field broadband coherent anti-Stokes Raman scattering microscopy

Chiara Ceconello; Federico Vernuccio; Arianna Bresci; Francesco Manetti; Salvatore Sorrentino; Renzo Vanna; Giulio Cerullo; Dario Polli

doi:10.1117/12.2650628

16 March 2023 Video-rate wide-field broadband coherent anti-Stokes Raman scattering microscopy

Chiara Ceconello, Federico Vernuccio, Arianna Bresci, Francesco Manetti, Salvatore Sorrentino, Renzo Vanna, Giulio Cerullo, Dario Polli

Author Affiliations +

Proceedings Volume 12390, High-Speed Biomedical Imaging and Spectroscopy VIII; 1239009 (2023) https://doi.org/10.1117/12.2650628
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

Coherent anti-Stokes Raman scattering is an extremely powerful non-linear optical (NLO) microscopy technique for label-free vibrational imaging allowing for chemical characterization of biological samples in their native state. We introduce here video-rate wide-field signal generation and acquisition over a large field of view (tens of micrometers) allowing for real-time investigation of fast biological dynamics. To this aim, our innovative approach employs an amplified femtosecond ytterbium laser source delivering high energy (≈ µJ) pulses in the near-infrared at 1035-nm central wavelength and 2-MHz repetition rate from which pump and Stokes beams are generated. Narrowband pump pulses with ≈1.1 nm bandwidth (10 cm⁻¹) guarantee sufficient spectral resolution for the vibrational Lorentian peaks. Broadband Stokes pulses in the 1100 to 1500 nm range are produced via supercontinuum generation in 10-mm YAG crystal and amplified in a non-collinear optical parametric amplifier (NOPA). This allows us to acquire hypercubes that cover the entire fingerprint region of the molecular vibrational spectrum, the richest in chemical information. Our results pave the way for future clinical diagnostics applications with video-rate imaging capabilities.

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Citation Download Citation

Chiara Ceconello, Federico Vernuccio, Arianna Bresci, Francesco Manetti, Salvatore Sorrentino, Renzo Vanna, Giulio Cerullo, and Dario Polli "Video-rate wide-field broadband coherent anti-Stokes Raman scattering microscopy", Proc. SPIE 12390, High-Speed Biomedical Imaging and Spectroscopy VIII, 1239009 (16 March 2023); https://doi.org/10.1117/12.2650628

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