Characterization of a microfluidic system with hydrodynamic focusing capabilities for flexible high-throughput analysis of microscopic particles using digital holographic microscopy

Álvaro Barroso; Mark Stude; Jian Kim; Steffi Ketelhut; Jürgen Schnekenburger; Björn Kemper

doi:10.1117/12.2649872

16 March 2023 Characterization of a microfluidic system with hydrodynamic focusing capabilities for flexible high-throughput analysis of microscopic particles using digital holographic microscopy

Álvaro Barroso, Mark Stude, Jian Kim, Steffi Ketelhut, Jürgen Schnekenburger, Björn Kemper

Author Affiliations +

Proceedings Volume 12391, Label-free Biomedical Imaging and Sensing (LBIS) 2023; 123910A (2023) https://doi.org/10.1117/12.2649872
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

Digital holographic microscopy (DHM) combined with microfluidics provides high-throughput label-free imaging flow cytometry for generating physical data from heterogeneous samples like particle or cell mixtures. For reliable analysis of the sample fluid, e.g., for cell counting, it is crucial that all particles in the sample fluid flow are monitored within the field of view of the optical microscope camera. In this work we combine a DHM system with a microfluidic system that allows lateral hydrodynamic focusing within a rectangular microfluidic channel. We investigate the hydrodynamic focusing effect at different settings of the microfluidic pump system by analyzing the retrieved DHM quantitative phase images of living cells flowing through the hydrodynamically-focused sample fluid flow. Our results show that DHM represents a feasible tool for the label-free characterization of sample fluid flows in microfluidic systems with hydrodynamic-focusing capabilities.

Conference Presentation

Citation Download Citation

Álvaro Barroso, Mark Stude, Jian Kim, Steffi Ketelhut, Jürgen Schnekenburger, and Björn Kemper "Characterization of a microfluidic system with hydrodynamic focusing capabilities for flexible high-throughput analysis of microscopic particles using digital holographic microscopy", Proc. SPIE 12391, Label-free Biomedical Imaging and Sensing (LBIS) 2023, 123910A (16 March 2023); https://doi.org/10.1117/12.2649872

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