Digital holographic microscopy for bacterial species classification and motility characterization

Jian Gao; Hadi Parsian; Jarl Carnahan; Gladys Alexandre; James Trolinger

doi:10.1117/12.2676170

4 October 2023 Digital holographic microscopy for bacterial species classification and motility characterization

Jian Gao, Hadi Parsian, Jarl Carnahan, Gladys Alexandre, James Trolinger

Proceedings Volume 12672, Applied Optical Metrology V; 126720C (2023) https://doi.org/10.1117/12.2676170
Event: SPIE Optical Engineering + Applications, 2023, San Diego, California, United States

Abstract

We have previously presented our work in developing and applying a commercial digital holographic microscopy (DHM) system for volumetric, 3D characterization of bacterial motility. The system was applied to simple biological systems, i.e., single bacterial species, to demonstrate its effectiveness. We are now applying DHM to more realistic conditions, including multiple bacterial types, to differentiate the species of interest for investigating their interactions. Our workflow for species classification and motility characterization combines DHM and machine learning. Specifically, our DHM instrument acquires holograms of single bacterial species and mixtures of different species, the software extracts in-focus images of individual bacteria and their trajectories, and deep convolutional neural network models are constructed and trained using the in-focus images and then deployed to the mixture data for classification. The motility and morphology of the predicted species in the mixture is consistent with the measurements from isolates, verifying the effectiveness of the developed workflow. This work showcases the application of DHM to investigate complex biological systems.

Conference Presentation

(2023) Published by SPIE. Downloading of the abstract is permitted for personal use only.

Citation Download Citation

Jian Gao, Hadi Parsian, Jarl Carnahan, Gladys Alexandre, and James Trolinger "Digital holographic microscopy for bacterial species classification and motility characterization", Proc. SPIE 12672, Applied Optical Metrology V, 126720C (4 October 2023); https://doi.org/10.1117/12.2676170

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