Spatially resolved SRS imaging of cells and tissues using circular polarization

Zhi Li; Hongje Jang; Yajuan Li; Anthony Fung; Yu Ping; Lingyan Shi

doi:10.1117/12.3008570

12 March 2024 Spatially resolved SRS imaging of cells and tissues using circular polarization

Zhi Li, Hongje Jang, Yajuan Li, Anthony Fung, Yu Ping, Lingyan Shi

Proceedings Volume 12847, Multiphoton Microscopy in the Biomedical Sciences XXIV; 128470R (2024) https://doi.org/10.1117/12.3008570
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

Stimulated Raman scattering (SRS) technique has been widely used in biomedical imaging, in which the CH band is usually used for detecting biomolecules. However, Raman peaks have the nonlinear identity limitation in which a Raman peak can be comprised of multiple molecules and a molecule can manifest itself in multiple Raman peaks. In this study, we applied a circularly polarized light for SRS (CP-SRS) imaging to examine the effect of polarization on differentiating CH vibrational modes. We observed that CP exerted a significant suppression on CH₂ bonds compared to CH₃ bonds, allowing for visualizing pure protein instead of protein-lipid complexes. This distinctive physics-informed unmixing method has the potential to enhance the chemical sensitivity of SRS imaging, displaying important implications in label-free biomedical imaging field.

(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.

Citation Download Citation

Zhi Li, Hongje Jang, Yajuan Li, Anthony Fung, Yu Ping, and Lingyan Shi "Spatially resolved SRS imaging of cells and tissues using circular polarization", Proc. SPIE 12847, Multiphoton Microscopy in the Biomedical Sciences XXIV, 128470R (12 March 2024); https://doi.org/10.1117/12.3008570

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available