Label-free position tracking in stuffed sparrow using phosphorescence

Tomohiko Hayakawa; Yuka Hiruma; Yushi Moko; Yushan Ke; Masatoshi Ishikawa

doi:10.1117/12.3005317

12 March 2024 Label-free position tracking in stuffed sparrow using phosphorescence

Tomohiko Hayakawa, Yuka Hiruma, Yushi Moko, Yushan Ke, Masatoshi Ishikawa

Proceedings Volume 12854, Label-free Biomedical Imaging and Sensing (LBIS) 2024; 1285403 (2024) https://doi.org/10.1117/12.3005317
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

To track living organisms, methods have been used such as spraying substances that easily produce phosphorescence or preparation at the genetic level; however, the need for advance preparation. Alternatively, it has become clear that RTP can be produced by excitation of organic materials with ultraviolet light. Since living organisms are composed of organic materials, phosphorescence is presumed to be generated. In this study, we will test this hypothesis and investigate its application to novel bioimaging without any preparation. Specifically, using a stuffed sparrow, we irradiate 375 nm excitation light to the feather area and take images using a high-speed, high-sensitivity camera. By measuring the phosphorescence lifetime after the end of the excitation light, we will track the phosphorescence that can be used for position tracking. In the experiment, the excitation light was actually irradiated on a stuffed sparrow, and the phosphorescene light as a label-free dynamic marker was tracked.

Conference Presentation

(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.

Citation Download Citation

Tomohiko Hayakawa, Yuka Hiruma, Yushi Moko, Yushan Ke, and Masatoshi Ishikawa "Label-free position tracking in stuffed sparrow using phosphorescence", Proc. SPIE 12854, Label-free Biomedical Imaging and Sensing (LBIS) 2024, 1285403 (12 March 2024); https://doi.org/10.1117/12.3005317

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