Utilizing DUET imaging for quantification of collagen and elastin as biomarkers for studying disease

Willy Ju; Samuel Border; Anupam Mitra; Eniola Okon; Hyunjae Jeong; Richard M. Levenson; Avi Rosenberg; Kuang-Yu Jen; Pinaki Sarder; Farzad Fereidouni

doi:10.1117/12.3009357

13 March 2024 Utilizing DUET imaging for quantification of collagen and elastin as biomarkers for studying disease

Willy Ju, Samuel Border, Anupam Mitra, Eniola Okon, Hyunjae Jeong, Richard M. Levenson, Avi Rosenberg, Kuang-Yu Jen, Pinaki Sarder, Farzad Fereidouni

Author Affiliations +

Proceedings Volume 12862, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications XV; 1286206 (2024) https://doi.org/10.1117/12.3009357
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

Collagen and elastin are prominent components in both normal and abnormal tissues, and their presence and distribution have great significance for fibrosis- and cancer-related processes. Collagen and elastin quantification in the context of fibrosis, often associated with irreparable organ injury, can predict the disease severity and patient prognosis. In the context of cancer, specific spatial collagen signatures are known to influence tumor microenvironments while identification of elastin is important in the context of treatment of metastatic cancers. Traditional methods to quantify collagen and elastin vary in accuracy, cost, and ease of use. Using DUET microscopy on H&E slides, high-resolution collagen and elastin mapping is possible without added staining steps or expensive optical instrumentation. We demonstrate this approach in chronic kidney disease (CKD), coronary artery disease (CAD), and for identifying vascular elastin in colon cancers.

Conference Presentation

(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.

Citation Download Citation

Willy Ju, Samuel Border, Anupam Mitra, Eniola Okon, Hyunjae Jeong, Richard M. Levenson, Avi Rosenberg, Kuang-Yu Jen, Pinaki Sarder, and Farzad Fereidouni "Utilizing DUET imaging for quantification of collagen and elastin as biomarkers for studying disease", Proc. SPIE 12862, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications XV, 1286206 (13 March 2024); https://doi.org/10.1117/12.3009357

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