The relentless rise of Multidrug-Resistant (MDR) bacteria significantly challenges global health, undermining the effectiveness of traditional antibiotics and demanding prompt development of new antimicrobial strategies. This study investigates the antimicrobial properties of Gamma-Aminobutyric Acid (GABA) silver nanoparticles (GABAAgNPs). GABAAgNPs were synthesized via photoreduction and subsequently characterized using UV-Vis spectroscopy, Transmission Electron Microscopy (TEM), and Zeta potential analysis. Their antimicrobial potential was assessed through a Minimum Inhibitory Concentration (MIC) assay. The photodynamic effect was studied under irradiation at the following wavelengths: 460 +/- 10 nm (2 min), 590 nm +/- 10 nm (2 min), and 660 nm +/- 10 nm for 2, 5, and 10 minutes. Our results demonstrated that GABAAgNPs induced porphyrin formation in E. coli, leading to microbial inhibition, further enhanced through the photodynamic effect. Thus, GABAAgNPs display dual functionality: directly targeting microbes while concurrently acting as photosensitizers in antimicrobial photodynamic inactivation (aPDI).
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