A digital holographic system for measuring bacterial growth in micro-fluidic chambers

Hussein Kamel; Julian Schmid; Moaaz R. Nizami; Igor Alekseenko; Florian Hausladen; Daniel Claus; Rainer Wittig; Damien P. Kelly

doi:10.1117/12.3022355

18 June 2024 A digital holographic system for measuring bacterial growth in micro-fluidic chambers

Hussein Kamel, Julian Schmid, Moaaz R. Nizami, Igor Alekseenko, Florian Hausladen, Daniel Claus, Rainer Wittig, Damien P. Kelly

Author Affiliations +

Proceedings Volume 12997, Optics and Photonics for Advanced Dimensional Metrology III; 129971G (2024) https://doi.org/10.1117/12.3022355
Event: SPIE Photonics Europe, 2024, Strasbourg, France

Conference Poster

Abstract

Digital holographic microscopy (DHM) has a wide variety of applications in biomedical sciences. Here we examine the use of an off-axis holographic system to monitor bacterial growth in commercially available ibidi micro-fluidic chambers. The imaged volume spans roughly 120 x 120 x 200 μm3, at 50x magnification. By use of an off-axis reference wave and some standard numerical filtering operations it is possible to recover the complex amplitude and thus the phase of the object wave. Two strains of bacteria are used in the research study: Escherichia coli and Staphylococcus warneri with dimensions around 1-4 microns. Examining these samples, showed that through the use of phase compensation techniques, the method can sustain an average Signal-to-Noise ratio (SNR) of around 5, allowing for the use of a simple thresholding algorithm for cell counting. In addition, the cell count dynamics determined using this method reflect the exponential increase of dilution steps very well, when compared against hypothetical cell counts. Details of the system operation, including a discussion of the signal processing routines, together with modifications to the general optic design of DHM systems are presented.

(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.

Citation Download Citation

Hussein Kamel, Julian Schmid, Moaaz R. Nizami, Igor Alekseenko, Florian Hausladen, Daniel Claus, Rainer Wittig, and Damien P. Kelly "A digital holographic system for measuring bacterial growth in micro-fluidic chambers", Proc. SPIE 12997, Optics and Photonics for Advanced Dimensional Metrology III, 129971G (18 June 2024); https://doi.org/10.1117/12.3022355

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