Noninvasive discrimination of prostate healthy and cancer cells by quantitative phase and Raman imaging

Hossein Khadem; Maria Antonietta Ferrara; Maria Mangini; Alberto Luini; Giuseppe Coppola; Anna Chiara De Luca

doi:10.1117/12.3017604

20 June 2024 Noninvasive discrimination of prostate healthy and cancer cells by quantitative phase and Raman imaging

Hossein Khadem, Maria Antonietta Ferrara, Maria Mangini, Alberto Luini, Giuseppe Coppola, Anna Chiara De Luca

Proceedings Volume 13006, Biomedical Spectroscopy, Microscopy, and Imaging III; 1300607 (2024) https://doi.org/10.1117/12.3017604
Event: SPIE Photonics Europe, 2024, Strasbourg, France

Abstract

In this study, we utilized polarization-sensitive holotomography and Raman imaging to analyze prostate healthy (PNT2) and cancer (PC3) cells treated with glucose. After 48-hour incubation, distinct morphological differences were observed in cancer cells, including changes in volume, number, and refractive index of lipid droplets (LDs). Raman imaging confirmed the glucose uptake of these LDs. Cancer cells exhibited larger and more numerous LDs, with higher mean refractive-index and birefringence, compared to healthy cells. The study achieved over 90% accuracy in discriminating between cell types, highlighting its potential in cancer diagnostics. The research contributes to biomedical spectroscopy, offering a valuable tool for understanding cancer cell morphology and enabling early and precise cancer detection.

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Citation Download Citation

Hossein Khadem, Maria Antonietta Ferrara, Maria Mangini, Alberto Luini, Giuseppe Coppola, and Anna Chiara De Luca "Noninvasive discrimination of prostate healthy and cancer cells by quantitative phase and Raman imaging", Proc. SPIE 13006, Biomedical Spectroscopy, Microscopy, and Imaging III, 1300607 (20 June 2024); https://doi.org/10.1117/12.3017604

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