Applications of two-photon fluorescence lifetime and second harmonic generation imaging technologies in pathological tissue sections

Wei Li; He Wang; Xiaoyu Li; Wei Yan; Yunyun Wang; Junle Qu

doi:10.1117/12.3030497

3 June 2024 Applications of two-photon fluorescence lifetime and second harmonic generation imaging technologies in pathological tissue sections

Wei Li, He Wang, Xiaoyu Li, Wei Yan, Yunyun Wang, Junle Qu

Author Affiliations +

Proceedings Volume 13182, 2024 International Conference on Optoelectronic Information and Optical Engineering (OIOE 2024); 131820M (2024) https://doi.org/10.1117/12.3030497
Event: 2024 International Conference on Optoelectronic Information and Optical Engineering (OIOE 2024), 2024, Kunming, China

Abstract

Two-photon fluorescence lifetime microscopy (TP-FLIM) is a powerful quantitative imaging technique that that can characterize and analyze the microenvironment changes of biological samples in the fluorescence lifetime dimension. It is not affected by factors such as excitation light intensity, sample bleaching, and fluorophore concentration, and has the advantages of high sensitivity and resolution. Therefore, it has extensive applications in biomedical research, disease diagnosis, and other fields. Second harmonic generation imaging (SHG) possesses the unique characteristics of high spatial resolution and imaging depth inherent in nonlinear optical imaging. SHG occurs in non-centrosymmetric structures, a property exhibited by collagen tissue in biological organisms. Consequently, SHG has been employed for specific tissue structure imaging. This paper combines TP-FLIM and SHG technologies to investigate pathological lung sections. Vector analysis of TP-FLIM is applied to analyze the fluorescence lifetime data of lung collagen fibers. Through TP-FLIM and SHG technologies, the lifetime of collagen fibers in the sample can be calculated and the collagen fiber tissue and non-collagen fiber tissue in the lung tissue sections can be clearly distinguished. This provides a new direction and possibility for indepth understanding of pulmonary fibrosis and other pathological mechanisms related to collagen fiber diseases, offering new insights into pathology and provisional diagnosis.

(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.

Citation Download Citation

Wei Li, He Wang, Xiaoyu Li, Wei Yan, Yunyun Wang, and Junle Qu "Applications of two-photon fluorescence lifetime and second harmonic generation imaging technologies in pathological tissue sections", Proc. SPIE 13182, 2024 International Conference on Optoelectronic Information and Optical Engineering (OIOE 2024), 131820M (3 June 2024); https://doi.org/10.1117/12.3030497

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available