Fluorescence line-narrowing spectroscopy in the study of chemical carcinogenesis

Ryszard Jankowiak; H. Jeong; Gerald J. Small

doi:10.1117/12.44245

1 July 1991 Fluorescence line-narrowing spectroscopy in the study of chemical carcinogenesis

Ryszard Jankowiak, H. Jeong, Gerald J. Small

Proceedings Volume 1435, Optical Methods for Ultrasensitive Detection and Analysis: Techniques and Applications; (1991) https://doi.org/10.1117/12.44245
Event: Optics, Electro-Optics, and Laser Applications in Science and Engineering, 1991, Los Angeles, CA, United States

Abstract

A laser spectroscopic technique known as 'fluorescence line narrowing spectroscopy' (FLNS) provides an unprecedented level of detail in the study of cellular macromolecular damage to DNA due to chemical reactions in vitro and in vivo with organic carcinogens. The reactions are believed to be the first step in the induction of tumors. Different stereoisomers of the metabolite benzo[a]pyrene diol epoxide (BPDE) which binds to DNA bases and the same stereoisomers in different DNA site configurations can be directly distinguished. By comparing BPDE adducts formed from synthetic polynucleotides of specific base composition, insights into the nature of BPDE-DNA adduct structure types can be obtained. It is shown that FLNS can be used to study metabolic pathways and DNA damage routes from benzo[a]pyrene and 7,12- dimethylbenzanthracene. BPDE-human hemoglobin adducts can also be identified. Due to its high sensitivity and superior selectivity, FLNS could be the basis for a practical and reliable body burden assessment methodology.

Citation Download Citation

Ryszard Jankowiak, H. Jeong, and Gerald J. Small "Fluorescence line-narrowing spectroscopy in the study of chemical carcinogenesis", Proc. SPIE 1435, Optical Methods for Ultrasensitive Detection and Analysis: Techniques and Applications, (1 July 1991); https://doi.org/10.1117/12.44245

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