Fluorescence imaging in photodynamic therapy

Alexander J. MacRobert; David Phillips

doi:10.1117/12.50461

1 December 1991 Fluorescence imaging in photodynamic therapy

Alexander J. MacRobert, David Phillips

Proceedings Volume 1439, International Conference on Scientific Optical Imaging; (1991) https://doi.org/10.1117/12.50461
Event: International Conference on Scientific Optical Imaging, 1990, Georgetown, Cayman Islands

Abstract

A quantitative technique based on fluorescence microscopic detection has been developed for measurements of photosensitizer distributions in sections of tissue and cultured cells. Imaging of photosensitizer fluorescence was achieved with negligible interference from sensitizer photodegradation and tissue autofluorescence using laser excitation and detection with a highly sensitive CCD (charge-coupled device) camera system. Techniques for the measurement of sensitizer fluorescence decay parameters in living biological cells are also described.

Citation Download Citation

Alexander J. MacRobert and David Phillips "Fluorescence imaging in photodynamic therapy", Proc. SPIE 1439, International Conference on Scientific Optical Imaging, (1 December 1991); https://doi.org/10.1117/12.50461

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available