Use of a cooled CCD camera for confocal light microscopy

Barry R. Masters

doi:10.1117/12.46260

1 June 1991 Use of a cooled CCD camera for confocal light microscopy

Barry R. Masters

Proceedings Volume 1448, Camera and Input Scanner Systems; (1991) https://doi.org/10.1117/12.46260
Event: Electronic Imaging '91, 1991, San Jose, CA, United States

Abstract

The use of a cooled CCD camera for confocal light microscopy provides several advantages. For low-light level applications such as reflected light microscopy from semi-transparent biological samples slow scan cooled CCD imaging devices provides an image with high dynamic range, linear response, and geometric stability of the pixels. These characteristics are required for accurate quantitative microscopy. The only cases where these detectors are unsuitable is when the temporal response of the image exceeds the readout time of the camera. For single shot events, even this limitation can be overcome with a partially masked CCD. The required spatial resolution of the object in the image plane is related to the spatial resolution of the camera detector. The detector should have twice the highest spatial resolution of the camera detector. The detector should have twice the highest spatial resolution of the object. In order to demonstrate the utility and application of a cooled CCD camera in microscopy, we have coupled a Photometrics cooled CCD camera containing a Tektronix 512, thinned, back illuminated CCD to a Technical Instruments, K2Bio confocal microscope. The high quality of the confocal images demonstrates the properties of the cooled CCD camera as a suitable detector for quantitative confocal microscopic imaging of weakly reflecting biological samples.

Citation Download Citation

Barry R. Masters "Use of a cooled CCD camera for confocal light microscopy", Proc. SPIE 1448, Camera and Input Scanner Systems, (1 June 1991); https://doi.org/10.1117/12.46260

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