Brain mapping by FTIR microspectroscopy

David L. Wetzel; Steven M. Le Vine

doi:10.1117/12.56461

1 March 1992 Brain mapping by FTIR microspectroscopy

David L. Wetzel, Steven M. Le Vine

Proceedings Volume 1575, 8th Intl Conf on Fourier Transform Spectroscopy; (1992) https://doi.org/10.1117/12.56461
Event: Eighth International Conference on Fourier Transform Spectroscopy, 1991, Lubeck-Travemunde, Germany

Abstract

Spatial resolution achieved with a state-of-the-art scanning molecular FT-IR microspectrometer allows excellent spectra to be obtained from relatively small areas of 8 micrometers thick section of brain tissue. Concentration mapping is achieved by picking absorbances or baseline-corrected absorbances of select peaks for each scan obtained at a particular x,y coordinate of the tissue section. Scanning was done in the transmission mode and a programmable microscope stage was used to position the specimen in the beam between scans. The chemical selectivity of the microspectroscopic technique allowed us to superimpose chemical data onto the spatially-defined structures, particularly myelin, in the brain. Since the chemical information is both qualitative and semiquantitative the heterogeneous distribution is revealed. Previously spectroscopic analysis was performed on myelin fractions obtained from brain homogenates. This did not allows observation of localized concentration differences, as is now the case. In addition to spectra, maps of selected key wavelength responses presented from frozen sections of normal brains, result in chemically selective distribution profiles in the gray matter, white matter and basal ganglia.

Citation Download Citation

David L. Wetzel and Steven M. Le Vine "Brain mapping by FTIR microspectroscopy", Proc. SPIE 1575, 8th Intl Conf on Fourier Transform Spectroscopy, (1 March 1992); https://doi.org/10.1117/12.56461

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available