FTIR spectroscopy, site-directed mutagenesis, and isotope labeling: a new approach for studying membrane proteins

Yi-Wu He; Mark P. Krebs; Judy Herzfeld; H. Gobind Khorana; Kenneth J. Rothschild

doi:10.1117/12.56283

1 March 1992 FTIR spectroscopy, site-directed mutagenesis, and isotope labeling: a new approach for studying membrane proteins

Yi-Wu He, Mark P. Krebs, Judy Herzfeld, H. Gobind Khorana, Kenneth J. Rothschild

Author Affiliations +

Proceedings Volume 1575, 8th Intl Conf on Fourier Transform Spectroscopy; (1992) https://doi.org/10.1117/12.56283
Event: Eighth International Conference on Fourier Transform Spectroscopy, 1991, Lubeck-Travemunde, Germany

Abstract

The FTIR absorption spectrum of a membrane protein contains bands due to the vibrations of individual chemical groups. Site-directed mutagenesis can be used to assign such bands to individual amino acid residues. Using this method, a band from the amino acid residue Tyr- 185 of bacteriorhodopsin, a light-driven proton pump, was identified in the low temperature FTIR absorption spectrum. Its intensity and frequency indicate that Tyr-185 exists in a partially deprotonated form in light-adapted bacteriorhodopsin.

Citation Download Citation

Yi-Wu He, Mark P. Krebs, Judy Herzfeld, H. Gobind Khorana, and Kenneth J. Rothschild "FTIR spectroscopy, site-directed mutagenesis, and isotope labeling: a new approach for studying membrane proteins", Proc. SPIE 1575, 8th Intl Conf on Fourier Transform Spectroscopy, (1 March 1992); https://doi.org/10.1117/12.56283

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available