Models for fluorescence energy transfer between moving donors and acceptors

B. Wieb Van der Meer; Matthew A. Raymer; Shawn L. Wagoner; Richard L. Hackney; Joseph M. Beechem; Enrico Gratton

doi:10.1117/12.58266

1 April 1992 Models for fluorescence energy transfer between moving donors and acceptors

B. Wieb Van der Meer, Matthew A. Raymer, Shawn L. Wagoner, Richard L. Hackney, Joseph M. Beechem, Enrico Gratton

Proceedings Volume 1640, Time-Resolved Laser Spectroscopy in Biochemistry III; (1992) https://doi.org/10.1117/12.58266
Event: OE/LASE '92, 1992, Los Angeles, CA, United States

Abstract

A number of theoretical models are introduced in which donors and acceptors perform lateral or rotational motion during the time in which fluorescence energy transfer takes place. Each case is reduced to an eigenvalue problem, and for each model calculations are made of observables, such as fluorescence intensities and anisotropies, by employing matrix methods. It is shown that the observables depend on the size of the motional step only if fluorescence energy transfer occurs. This finding indicates that fluorescence energy transfer studies may reveal whether the dynamics of a system (e.g., a protein) is better described in terms of transitions between a number of discrete states or in terms of diffusion equations. Some theoretical tools are offered for analyzing fluorescence energy transfer data without restrictive assumptions for motional averaging regimes or the orientation factor.

Citation Download Citation

B. Wieb Van der Meer, Matthew A. Raymer, Shawn L. Wagoner, Richard L. Hackney, Joseph M. Beechem, and Enrico Gratton "Models for fluorescence energy transfer between moving donors and acceptors", Proc. SPIE 1640, Time-Resolved Laser Spectroscopy in Biochemistry III, (1 April 1992); https://doi.org/10.1117/12.58266

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