Time-resolved fluorescence analysis

J. B. Alexander Ross; William R. Laws

doi:10.1117/12.137726

14 August 1992 Time-resolved fluorescence analysis

J. B. Alexander Ross, William R. Laws

Proceedings Volume 1681, Optically Based Methods for Process Analysis; (1992) https://doi.org/10.1117/12.137726
Event: SPIE's 1992 Symposium on Process Control and Monitoring, 1992, Somerset, NJ, United States

Abstract

The choice of fluorescence as an analytical tool is often based on its intrinsic sensitivity. Compounds can be identified on the basis of their steady-state excitation and emission spectra. Resolution and information can be enhanced by the parameters obtained from time-resolved fluorescence. This includes both the intensity decay (lifetimes) and anisotropy decay parameters. For example, often substances have overlapping steady-state excitation and emission spectra. Thus, they can be difficult to resolve. However, these same compounds will often have different fluorescence lifetimes. This allows resolution by excited-state decay kinetics. By testing various associations of the intensity decay parameters with the excitation and emission parameters, as well as with other experimental variables such as pH, temperature, pressure, viscosity, or interaction with other solutes (such as collisional quenchers), one can obtain substantial information about the physical and chemical nature of the sample. In this way, one may 'finger print' complex mixtures, assess compound purity, and characterize a fluorescent compound.

Citation Download Citation

J. B. Alexander Ross and William R. Laws "Time-resolved fluorescence analysis", Proc. SPIE 1681, Optically Based Methods for Process Analysis, (14 August 1992); https://doi.org/10.1117/12.137726

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available