Effect of laser irradiation on the interaction of hematoporphyrin with physiological ions

Libusa Sikurova; L. V. Zhorina; B. Cunderlikova

doi:10.1117/12.146194

17 June 1993 Effect of laser irradiation on the interaction of hematoporphyrin with physiological ions

Libusa Sikurova, L. V. Zhorina, B. Cunderlikova

Proceedings Volume 1922, Laser Study of Macroscopic Biosystems; (1993) https://doi.org/10.1117/12.146194
Event: Laser Spectroscopy of Biomolecules: 4th International Conference on Laser Applications in Life Sciences, 1992, Jyvaskyla, Finland

Abstract

The effect of KCl ions on hematoporphyrin (HP) and its binding to DOPC liposomes were investigated. The absorption spectra of HP (2 - 30 micrometers ol/l) in KCl solutions (0.1 - 1.0 mol/l) showed two intensive peaks at 390 nm (monomer peak) and at 374 nm (dimer peak). When these samples were kept in the dark an agreement with HP aqueous spectra was observed. He-Ne laser (632 nm, 1 mW) illumination of the dye in KCl solution resulted in a change of the spectral appearance: both the original absorption bands became less distinguishable up to their disappearing and a new band at 404 nm was found. Its intensity was systematically enhanced as the time of illumination was increased (5 - 40 min.). This effect can be attributed to the laser energy mediated interaction of HP with ions.

Citation Download Citation

Libusa Sikurova, L. V. Zhorina, and B. Cunderlikova "Effect of laser irradiation on the interaction of hematoporphyrin with physiological ions", Proc. SPIE 1922, Laser Study of Macroscopic Biosystems, (17 June 1993); https://doi.org/10.1117/12.146194

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available