Raman microspectroscopy and atomic force microscopy of chromosomal banding patterns

Gerwin J. Puppels; Constant A.J. Putman; Bart G. de Grooth; Jan Greve

doi:10.1117/12.146192

17 June 1993 Raman microspectroscopy and atomic force microscopy of chromosomal banding patterns

Gerwin J. Puppels, Constant A.J. Putman, Bart G. de Grooth, Jan Greve

Proceedings Volume 1922, Laser Study of Macroscopic Biosystems; (1993) https://doi.org/10.1117/12.146192
Event: Laser Spectroscopy of Biomolecules: 4th International Conference on Laser Applications in Life Sciences, 1992, Jyvaskyla, Finland

Abstract

The potentials and first results of a combined Raman microspectroscopic and atomic force microscopic study of the banding pattern of polytene chromosomes are described. The results of Raman experiments indicate that differences in DNA content between bands and interbands are not as large as previously suggested in the literature. By means of atomic force microscopy the ultrastructure of chromosome bands and interbands can be analyzed.

Citation Download Citation

Gerwin J. Puppels, Constant A.J. Putman, Bart G. de Grooth, and Jan Greve "Raman microspectroscopy and atomic force microscopy of chromosomal banding patterns", Proc. SPIE 1922, Laser Study of Macroscopic Biosystems, (17 June 1993); https://doi.org/10.1117/12.146192

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