Laser-triggered releasing of fluorescein from thermosensible liposomes: a new method for quantification of laser-induced photocoagulation

Serge R. Mordon; Thomas Desmettre; Jean-Marie Devoisselle; G. Constantinides; Jean Marc Brunetaud

doi:10.1117/12.182943

17 August 1994 Laser-triggered releasing of fluorescein from thermosensible liposomes: a new method for quantification of laser-induced photocoagulation

Serge R. Mordon, Thomas Desmettre, Jean-Marie Devoisselle, G. Constantinides, Jean Marc Brunetaud

Author Affiliations +

Proceedings Volume 2134, Laser-Tissue Interaction V; and Ultraviolet Radiation Hazards; (1994) https://doi.org/10.1117/12.182943
Event: OE/LASE '94, 1994, Los Angeles, CA, United States

Abstract

The objective of this in-vivo study was to assess the possible use of temperature sensitive liposomes in an established model such as liver as a new approach to monitor the temperature induced by a laser. Temperature sensitive liposomes (DSPC: distearoylphosphatidylcholine) loaded with carboxyfluorescein were prepared by the Bancham procedure. These liposomes (1 ml solution) were injected into adult male wistar rats. Two hours later, the liver was exposed and irradiated with a 100 W Nd:YAG laser using pulses lasting from 100 ms to 260 ms. Simultaneously, the surface temperature was recorded with a thermographic camera. The fluorescence emission was measured with a fluorescent imaging system. The results show that the dye is released in response to laser energy. The amount of the drug release increases linearly with increasing temperature in the range 45 degree(s)C - 60 degree(s)C. Moreover, the release occurs in a short period of time upon brief exposure to its phase transition temperature. The temperature range could be modified and adapted by using different liposomes formulation, for example DPPC (dipalmitoylphosphatidylcholine) for lower temperature (35 - 45 degree(s)C) or DSPE (distearoylphosphatidylethanolamine) for higher temperature (65 - 75 degree(s)C).

Citation Download Citation

Serge R. Mordon, Thomas Desmettre, Jean-Marie Devoisselle, G. Constantinides, and Jean Marc Brunetaud "Laser-triggered releasing of fluorescein from thermosensible liposomes: a new method for quantification of laser-induced photocoagulation", Proc. SPIE 2134, Laser-Tissue Interaction V; and Ultraviolet Radiation Hazards, (17 August 1994); https://doi.org/10.1117/12.182943

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
10 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Temperature metrology

Laser coagulation

Liver

Cameras

Imaging systems

In vivo imaging

Luminescence

Show All Keywords

Keywords/Phrases

Search In:

Publication Years