Monitoring the surface adsorption of cyanine dyes via evanescent wave-excited fluorescence

Robert J. Obremski; Keira Semones; John W. Silzel

doi:10.1117/12.181359

27 July 1994 Monitoring the surface adsorption of cyanine dyes via evanescent wave-excited fluorescence

Robert J. Obremski, Keira Semones, John W. Silzel

Proceedings Volume 2138, Longer Wavelength Lasers and Applications; (1994) https://doi.org/10.1117/12.181359
Event: OE/LASE '94, 1994, Los Angeles, CA, United States

Abstract

By confining light from a diode laser within a solid planar waveguide in contact with a liquid sample, an electromagnetic field is produced which extends into the solution for a distance of several hundred nanometers. This `evanescent wave' has all the properties (e.g. power, wavelength, etc.) of light propagating freely through the solution, and as such can excite fluorescence if suitable fluorophores are present. The evanescent wave technique has the advantage of being confined to the very small volume found within 100 - 200 nm of the waveguide surface, so that molecules in the bulk solution, away from the waveguide, do not experience the evanescent wave field. Evanescent wave excited fluorescence is therefore a spatially-specific probe of surface phenomena at the liquid-solid interface.

Citation Download Citation

Robert J. Obremski, Keira Semones, and John W. Silzel "Monitoring the surface adsorption of cyanine dyes via evanescent wave-excited fluorescence", Proc. SPIE 2138, Longer Wavelength Lasers and Applications, (27 July 1994); https://doi.org/10.1117/12.181359

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