Ultrathin antibody networks for detection of antigens

Andreas Hartmann; Daniel Bock; Michael Martin; Stefan Seeger

doi:10.1117/12.201251

10 February 1995 Ultrathin antibody networks for detection of antigens

Andreas Hartmann, Daniel Bock, Michael Martin, Stefan Seeger

Proceedings Volume 2331, Medical Sensors II and Fiber Optic Sensors; (1995) https://doi.org/10.1117/12.201251
Event: International Symposium on Biomedical Optics Europe '94, 1994, Lille, France

Abstract

Most affinity sensor principles demand immobilized receptor molecules. A widely used multiple-step technique for covalent immobilization of proteins is based on a silanization procedure and a crosslinking agent, e.g., glutardialdehyde. In the presented work non- amphophilic Langmuir-Blodgett films based on phthalocy-aninato-polysiloxane derivatives or copolyglutamates are used to immobilize antibodies as monolayers. Due to a photopolymerization process of terminal C-C-double bonds at alkylic side chains of the non- amphophilic substance the stability of the films is improved compared to conventional LB films. Antibodies can be immobilized by a one-step-method subsequent to illumination. Polymethyl-methacrylate (PMMA) fibers are found to be ideal substrates for the antibody transfer. Enzyme-linked immunosorbent assays (ELISA) give quite good specificities and densities of active immobilized immunoglobulin (IgG). For antigen detection these films are combined with compact evanescent wave sensors. Antibodies were immobilized at the surface of a waveguide using the Langmuir-Blodgett (LB) technique. The light source is a diode laser, which results in a small and compact set-up and due to the red excitation wavelength the background fluorescence is reduced.

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Andreas Hartmann, Daniel Bock, Michael Martin, and Stefan Seeger "Ultrathin antibody networks for detection of antigens", Proc. SPIE 2331, Medical Sensors II and Fiber Optic Sensors, (10 February 1995); https://doi.org/10.1117/12.201251

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