Antibody-based fluorometric assay for detection of the explosives TNT and PETN

Linda L. Judd; Anne W. Kusterbeck; David W. Conrad; Hao Yu; Herbert L. Myles Jr.; Frances S. Ligler

doi:10.1117/12.208478

8 May 1995 Antibody-based fluorometric assay for detection of the explosives TNT and PETN

Linda L. Judd, Anne W. Kusterbeck, David W. Conrad, Hao Yu, Herbert L. Myles Jr., Frances S. Ligler

Proceedings Volume 2388, Advances in Fluorescence Sensing Technology II; (1995) https://doi.org/10.1117/12.208478
Event: Photonics West '95, 1995, San Jose, CA, United States

Abstract

Advances in long wavelength fluorophore development have reduced interference from the naturally occurring background fluorescence often present in environmental samples, permitting significant progress in explosive and environmental sensing. The Center for Bio/Molecular Science and Engineering at the Naval Research Laboratory has developed a fluorometric, antibody-based, continuous flow immunosensor which can detect nanogram quantities of small molecular weight molecules such as trinitrotoluene and pentaerythritol tetranitrate. In the flow immunosensor, antibodies are immobilized onto a solid support matrix, exposed to a fluorescently tagged analyte, and placed into a small disposable column connected to an aqueous flow stream. Upon sample introduction, an amount of the fluorescently labelled analyte is displaced that is proportional to the concentration of unlabelled analyte present in the sample. The intrinsic nature of anti-body antigen binding also provides the unit with an inherently high degree of sensitivity and specificity. A positive signal can be generated in under 2 minutes, allowing quick analysis of numerous samples with minimal data handling. Development of a single column assay for the detection of both explosives has decreased both detection time and cost per assay. Cross reactivity studies and studies investigating interference from intrinsic fluorescence in several media have also been conducted.

Citation Download Citation

Linda L. Judd, Anne W. Kusterbeck, David W. Conrad, Hao Yu, Herbert L. Myles Jr., and Frances S. Ligler "Antibody-based fluorometric assay for detection of the explosives TNT and PETN", Proc. SPIE 2388, Advances in Fluorescence Sensing Technology II, (8 May 1995); https://doi.org/10.1117/12.208478

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