Sensitive laser wave-mixing detection methods for biomedical applications

Jon A. Nunes; Sandrine Berniolles; William M. Tong

doi:10.1117/12.208479

8 May 1995 Sensitive laser wave-mixing detection methods for biomedical applications

Jon A. Nunes, Sandrine Berniolles, William M. Tong

Proceedings Volume 2388, Advances in Fluorescence Sensing Technology II; (1995) https://doi.org/10.1117/12.208479
Event: Photonics West '95, 1995, San Jose, CA, United States

Abstract

Four-wave mixing is presented as an ultrasensitive detection method for liquid analytes in capillary flow cells and for circular dichroism measurements. A two-input-beam forward- scattering degenerate four-wave mixing optical setup offers simplified optical alignment, effective use of compact low-power lasers, and convenient interface to powerful chemical separation methods using capillary columns. The use of a single focusing lens for both input beams provides very high wave-mixing efficiency and tight focusing inside a small detection probe volume. Attomole or better detection sensitivity levels of this nonlinear laser detector compare favorably to those of many conventional laser-based analytical methods, yet this nonlinear detector can detect both fluorescing and non-fluorescing analytes. One can conveniently interface this multi-wave mixing detector to liquid chromatography and capillary electrophoresis for on-column detection. Nonlinear wave-mixing as a sensitive `direct' or `indirect' absorbance detection method offers important advantages in many biomedical applications.

Citation Download Citation

Jon A. Nunes, Sandrine Berniolles, and William M. Tong "Sensitive laser wave-mixing detection methods for biomedical applications", Proc. SPIE 2388, Advances in Fluorescence Sensing Technology II, (8 May 1995); https://doi.org/10.1117/12.208479

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