Two-dimensional infrared fluorescence scanner used for DNA analysis

Lyle Richard Middendorf; Robert C. Bruce; John A. Brumbaugh; Daniel L. Grone; Gi Y. Jang; Peter Richterich; Holges Joachim Hoeltke; Richard J. Williams; Mauro Dr. Peralta

doi:10.1117/12.208522

8 May 1995 Two-dimensional infrared fluorescence scanner used for DNA analysis

Lyle Richard Middendorf, Robert C. Bruce, John A. Brumbaugh, Daniel L. Grone, Gi Y. Jang, Peter Richterich, Holges Joachim Hoeltke, Richard J. Williams, Mauro Dr. Peralta

Author Affiliations +

Proceedings Volume 2388, Advances in Fluorescence Sensing Technology II; (1995) https://doi.org/10.1117/12.208522
Event: Photonics West '95, 1995, San Jose, CA, United States

Abstract

A LI-COR Model 4000 DNA Sequencer has been modified by removing the internal scanning infrared fluorescence microscope and combining it with an external, orthogonal scanner. Due to the reduced background fluorescence and light scattering of nylon membranes in the near- infrared (8000 nm) as compared to the visible region of the optical spectrum, sensitivity of labeled DNA fragments is enhanced. Dot blots of dilution series of labeled oligonucleotides reveal a detection limit of 25 attomole (25 X 10^-18 mole). DNA fragments blotted onto nylon membranes using direct transfer electrophoresis in multiplex DNA sequencing can also be detected and subsequently analyzed.

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Lyle Richard Middendorf, Robert C. Bruce, John A. Brumbaugh, Daniel L. Grone, Gi Y. Jang, Peter Richterich, Holges Joachim Hoeltke, Richard J. Williams, and Mauro Dr. Peralta "Two-dimensional infrared fluorescence scanner used for DNA analysis", Proc. SPIE 2388, Advances in Fluorescence Sensing Technology II, (8 May 1995); https://doi.org/10.1117/12.208522

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