Extracting actin fibers in 3D cell fluorescence microscope images

Ping Fu; Steven A. Shafer; Raul E. Valdes-Perez

doi:10.1117/12.208747

12 May 1995 Extracting actin fibers in 3D cell fluorescence microscope images

Ping Fu, Steven A. Shafer, Raul E. Valdes-Perez

Proceedings Volume 2434, Medical Imaging 1995: Image Processing; (1995) https://doi.org/10.1117/12.208747
Event: Medical Imaging 1995, 1995, San Diego, CA, United States

Abstract

Extracting 3-D position of actin fibers is an important task in bio-medical research, since it can lead to a quantitative description of cell structure, motion and development. In this paper, we present a new approach to automatically extract these fibers from a 3-D stack of cell images obtained by three-dimensional fluorescence microscopy. Our algorithm incorporates two stages. First, the computer vision technique of depth-from-focusing is used to construct the 2-D best focused pseudo-image and the depth map from the original images. Second, we track actin fibers using the 2-D pseudo-image and the depth map based on a dense local filtering strategy and continuity constraints. A group of specially designed local line feature filters and continuity constraints are used to guide the tracking. Also we applied a backtracking strategy to refine the fiber searching when ambiguities occur. Finally, the systematic method of searching for all fibers in an image is presented.

Citation Download Citation

Ping Fu, Steven A. Shafer, and Raul E. Valdes-Perez "Extracting actin fibers in 3D cell fluorescence microscope images", Proc. SPIE 2434, Medical Imaging 1995: Image Processing, (12 May 1995); https://doi.org/10.1117/12.208747

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