Design of environment-responsive biomolecular systems

Masuo Aizawa; T. Niimi; T. Haruyama; E. Kobatake

doi:10.1117/12.232133

9 February 1996 Design of environment-responsive biomolecular systems

Masuo Aizawa, T. Niimi, T. Haruyama, E. Kobatake

Proceedings Volume 2716, Smart Structures and Materials 1996: Smart Materials Technologies and Biomimetics; (1996) https://doi.org/10.1117/12.232133
Event: 1996 Symposium on Smart Structures and Materials, 1996, San Diego, CA, United States

Abstract

Two different types of biomolecular network systems have been designed to respond to the environmental conditions. One is the calmodulin and enzyme (phosphodiesterase, PDE) that activates phosphodiesterase through the conformational change in responding calcium ion. Calmodulin was genetically engineered to be fused with glutathione-S-transferase (GST). Calmodulin/GST fused protein was self-assembled on the gold surface through glutathione. The calmodulin/GST protein layer exhibited an ability to modulate the PDE activity in a solution phase depending on the calcium ion concentration. The other is the engineered gene structure that produces firefly luciferase in responding environmental pollutants. A TOL plasmid, encoding a binding protein xyl R for xyline and a marker enzyme firefly luciferase, has been implemented in a bacterial cell. The whole cell responded to environmentally hazardous substances such as xylene in emitting light.

Citation Download Citation

Masuo Aizawa, T. Niimi, T. Haruyama, and E. Kobatake "Design of environment-responsive biomolecular systems", Proc. SPIE 2716, Smart Structures and Materials 1996: Smart Materials Technologies and Biomimetics, (9 February 1996); https://doi.org/10.1117/12.232133

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