NADH fluorescence/UV reflectance ratio provides a semi-quantitative measure for NADH fluorometry of blood-perfused rat heart

J. M. C. C. Coremans; C. Ince; Hajo A. Bruining; Gerwin J. Puppels

doi:10.1117/12.260640

11 December 1996 NADH fluorescence/UV reflectance ratio provides a semi-quantitative measure for NADH fluorometry of blood-perfused rat heart

J. M. C. C. Coremans, C. Ince, Hajo A. Bruining, Gerwin J. Puppels

Author Affiliations +

Proceedings Volume 2927, Optical and Imaging Techniques for Biomonitoring II; (1996) https://doi.org/10.1117/12.260640
Event: BiOS Europe '96, 1996, Vienna, Austria

Abstract

A procedure for semi-quantitative analysis of NADH fluorescence image is presented which enables non-invasive monitoring of the metabolic state of tissue in vivo. In blood perfused tissue, the NADH fluorescence intensity can be disturbed by movement and by hemodynamic and oximetric effects. These factors alter the tissue absorbance of UV excitation light and thereby the NADH fluorescence excitation efficiency. Combination of the theories of front- face fluorimetry and Kubelka and Munk for description of NADH fluorescence (F_NADH) and UV reflectance (R₃₆₅^d) of optically thick samples, predicts that the F_NADH/R₃₆₅^d ratio provides adequate compensation for changes in tissue absorbance. The validity of this correction procedure is verified in tissue phantoms, in Langendorff perfused rat hearts and for rat hearts in vivo. Movement artifacts were eliminated using a CCD video camera with a biprism configuration for simultaneous measurement of fluorescence and reflectance images. Therefore, the F_NADH/R^d ratio can be used to monitor the metabolic state of blood perfused tissue.

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J. M. C. C. Coremans, C. Ince, Hajo A. Bruining, and Gerwin J. Puppels "NADH fluorescence/UV reflectance ratio provides a semi-quantitative measure for NADH fluorometry of blood-perfused rat heart", Proc. SPIE 2927, Optical and Imaging Techniques for Biomonitoring II, (11 December 1996); https://doi.org/10.1117/12.260640

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