Autofluorescence of normal and neoplastic human brain tissue: an aid for intraoperative delineation of tumor resection margins

Giovanni F. Bottiroli; Anna Cleta Croce; Donata Locatelli; Rosanna Nano; Ermanno Giombelli; Alberto Messina; Eugenio Benericetti

doi:10.1117/12.297933

13 January 1998 Autofluorescence of normal and neoplastic human brain tissue: an aid for intraoperative delineation of tumor resection margins

Giovanni F. Bottiroli, Anna Cleta Croce, Donata Locatelli, Rosanna Nano, Ermanno Giombelli, Alberto Messina, Eugenio Benericetti

Author Affiliations +

Proceedings Volume 3196, Optical and Imaging Techniques for Biomonitoring III; (1998) https://doi.org/10.1117/12.297933
Event: BiOS Europe '97, 1997, San Remo, Italy

Abstract

Light-induced autofluorescence measurements were made on normal and tumor brain tissues to assess their spectroscopic properties and to verify the potential of this parameter for an intraoperative delineation of tumor resection margins. Spectrofluorometric analysis was performed both at the microscope on tissue sections from surgical resection, and on patients affected by glioblastoma, during surgical operation. Significant differences in autofluorescence emission properties were found between normal and tumor tissues in both ex vivo and in vivo measurements, indicating that the lesion can be distinguished from the informal surrounding tissues by the signal amplitude and the spectral shape. The non-invasiveness of the technique opens interesting prospects for improving the efficacy of neurosurgical operation, by allowing an intraoperative delimitation of tumor resection margins.

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Giovanni F. Bottiroli, Anna Cleta Croce, Donata Locatelli, Rosanna Nano, Ermanno Giombelli, Alberto Messina, and Eugenio Benericetti "Autofluorescence of normal and neoplastic human brain tissue: an aid for intraoperative delineation of tumor resection margins", Proc. SPIE 3196, Optical and Imaging Techniques for Biomonitoring III, (13 January 1998); https://doi.org/10.1117/12.297933

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