Organ transplant tissue rejection: detection and staging by fluorescence spectroscopy

Calum E. MacAulay; Peter D. Whitehead; Bruce McManus; Haishan Zeng; Janet Wilson-McManus; Nick MacKinnon; David C. Morgan; Chunming Dong; Paul Gerla; Jennifer Kenyon

doi:10.1117/12.312312

1 July 1998 Organ transplant tissue rejection: detection and staging by fluorescence spectroscopy

Calum E. MacAulay, Peter D. Whitehead, Bruce McManus, Haishan Zeng, Janet Wilson-McManus, Nick MacKinnon, David C. Morgan, Chunming Dong, Paul Gerla, Jennifer Kenyon

Author Affiliations +

Proceedings Volume 3245, Lasers in Surgery: Advanced Characterization, Therapeutics, and Systems VIII; (1998) https://doi.org/10.1117/12.312312
Event: BiOS '98 International Biomedical Optics Symposium, 1998, San Jose, CA, United States

Abstract

Patients receiving heart or other organ transplants usually require some level of anti-rejection drug therapy, most commonly cyclosporine. The rejection status of the organ must be monitored to determine the optimal anti-rejection drug therapy. The current method for monitoring post-transplant rejection status of heart transplant patients consists of taking biopsies from the right ventricle. In this work we have developed a system employing optical and signal-processing techniques that will allow a cardiologist to measure spectral changes associated with tissue rejection using an optical catheter probe. The system employs time gated illumination and detection systems to deal with the dynamic signal acquisition problems associated with in vivo measurements of a beating heart. Spectral data processing software evaluates and processes the data to produce a simple numerical score. Results of measurements made on 100 excised transplanted isograft and allograft rat hearts have demonstrated the ability of the system to detect the presence of rejection and to accurately correlate the spectroscopic results with the ISHLT (International Society for Heart and Lung Transplantation) stage of rejection determined by histopathology. In vivo measurements using a pig transplant model are now in process.

Citation Download Citation

Calum E. MacAulay, Peter D. Whitehead, Bruce McManus, Haishan Zeng, Janet Wilson-McManus, Nick MacKinnon, David C. Morgan, Chunming Dong, Paul Gerla, and Jennifer Kenyon "Organ transplant tissue rejection: detection and staging by fluorescence spectroscopy", Proc. SPIE 3245, Lasers in Surgery: Advanced Characterization, Therapeutics, and Systems VIII, (1 July 1998); https://doi.org/10.1117/12.312312

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available