In vivo infrared and Raman spectroscopy of human stratum corneum

Gerald W. Lucassen; Peter J. Caspers; Gerwin J. Puppels

doi:10.1117/12.306105

24 April 1998 In vivo infrared and Raman spectroscopy of human stratum corneum

Gerald W. Lucassen, Peter J. Caspers, Gerwin J. Puppels

Proceedings Volume 3257, Infrared Spectroscopy: New Tool in Medicine; (1998) https://doi.org/10.1117/12.306105
Event: BiOS '98 International Biomedical Optics Symposium, 1998, San Jose, CA, United States

Abstract

ATR-FTIR spectroscopy and Raman spectroscopy were employed to obtain information about the molecular composition and hydration of skin in vivo. Both techniques enable the in vivo acquisition of high quality spectra within 10-30s at a spectral resolution of 8cm^-1. The penetration depth of ATR-FTIR is about 1.5 (Mu) m. Raman spectra could be obtained with a resolution of about 5 micrometers . ATR-FTIR spectra of hydrated stratum corneum were analyzed using a band fitting algorithm. By means of this algorithm the signal contributions of water relative to protein signal contributions could be determined. The results of Raman microspectroscopic experiments on frozen sections and isolated skin components were used for the interpretation of Raman spectra obtained in vivo. Information was obtained about lipid components present in the stratum corneum. These were shown to vary widely between individuals and between different locations on the body. The combination of these spectroscopic techniques may prove to be valuable for applications in dermatology and skin care.

Citation Download Citation

Gerald W. Lucassen, Peter J. Caspers, and Gerwin J. Puppels "In vivo infrared and Raman spectroscopy of human stratum corneum", Proc. SPIE 3257, Infrared Spectroscopy: New Tool in Medicine, (24 April 1998); https://doi.org/10.1117/12.306105

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