New optoelectronic system based on the surface plasmon resonance phenomenon: application to the concentration determination of DD-fragment of fibrinogen

Ekaterina V. Kostyukevich; Boris A. Snopok; Stepan A. Zinio; Yuri M. Shirshov; Irina N. Kolesnikova; Edward V. Lugovskoi

doi:10.1117/12.323542

24 September 1998 New optoelectronic system based on the surface plasmon resonance phenomenon: application to the concentration determination of DD-fragment of fibrinogen

Ekaterina V. Kostyukevich, Boris A. Snopok, Stepan A. Zinio, Yuri M. Shirshov, Irina N. Kolesnikova, Edward V. Lugovskoi

Author Affiliations +

Proceedings Volume 3414, Opto-Contact: Workshop on Technology Transfers, Start-Up Opportunities,and Strategic Alliances; (1998) https://doi.org/10.1117/12.323542
Event: Lasers and Materials in Industry and Opto-Contact Workshop, 1998, Quebec, Canada

Abstract

The interaction of DD-fragment of fibrinogen with antibodies immobilized on the surface of polycrystalline gold films monoclonal has been studied by means of Surface Plasmon Resonance (SPR) biosensor instrument as a model system for real-time biomolecular interaction analysis. A BioHelper Plasmon-002 device (ISP NASU, Kiev, Ukraine), that exploits the SPR phenomenon to detect the interactions between sensor layer and analyte has been used for investigation. The results show that the kinetic parameters of antigen-antibody interactions are directly related to concentration of DD- fragment of fibrinogen in the examined solutions in the range from 10² to 10⁵ ng/ml. The binding kinetic data can be described by a set of several time-division binding equilibrium, each of them correlated well with an ideal binding first-order reactions. The influence of the procedure of antibody immobilization on the quasi flat gold surface has been examined as well.

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Ekaterina V. Kostyukevich, Boris A. Snopok, Stepan A. Zinio, Yuri M. Shirshov, Irina N. Kolesnikova, and Edward V. Lugovskoi "New optoelectronic system based on the surface plasmon resonance phenomenon: application to the concentration determination of DD-fragment of fibrinogen", Proc. SPIE 3414, Opto-Contact: Workshop on Technology Transfers, Start-Up Opportunities,and Strategic Alliances, (24 September 1998); https://doi.org/10.1117/12.323542

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